Stimulatory Effect of Indole – 3 – Butyric Acid and Rooting Media on Adventitious Rooting in Epipremnum Aureum ‘Marble Queen’ Stem Cuttings

Attanayake R.M.T.D; Dasanayaka Y.M.H.M.H; Dilshan H.K.L; Hettiarachchi W.A.B.H; Sandarenu K.M.S.D; Gamage S.N.W; Wickramasinghe P.A.S.C; Dayarathna C.R; Kumarihami H.M.P.C

doi:10.30997/ijar.v4i3.360

Attanayake R.M.T.D Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka.
Dasanayaka Y.M.H.M.H Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka
Dilshan H.K.L Mike Flora (Pvt) Ltd., Rambukkana, Sri Lanka
Hettiarachchi W.A.B.H Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka
Sandarenu K.M.S.D Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka
Gamage S.N.W Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka
Wickramasinghe P.A.S.C Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka
Dayarathna C.R Mike Flora (Pvt) Ltd., Rambukkana, Sri Lanka
Kumarihami H.M.P.C Mike Flora (Pvt) Ltd., Rambukkana, Sri Lanka

DOI: https://doi.org/10.30997/ijar.v4i3.360

Keywords: cuttings, indole – 3 – butyric acid, marble queen, propagation, rooting media

Abstract

Epipremnum aureum 'Marble Queen' has high demand in the floriculture industry and is propagated commercially using single nodal stem cuttings. In the floriculture industry, they are exported as rooted and unrooted cuttings. Two experiments were conducted to determine the rooting performance of 'Marble Queen' cuttings for both rooted and unrooted export purposes. Experiment one tested the rooting performances of unrooted cuttings after an export simulation (48-hour cold storage at 21°C). In experiment two, the effect of rooting media and IBA was evaluated to produce rooted cuttings. Two different rooting media (coir dust and oasis) and four different concentrations of IBA (0 mg·L^-1, 250 mg·L^-1, 500 mg·L^-1, and 1000 mg·L^-1) were tested. Rooting media showed the most significant effects on the rooting of 'Marble Queen' cuttings. Coir dust medium reported the highest number of roots per cutting, root-to-shoot ratio, and average root diameter. Minimum days to bud and root initiation and a higher number of leaf buds were observed in the oasis medium. Early rooting and a higher number of roots were observed with the application of 500 mg·L^-1 IBA. The use of an appropriate rooting medium and optimum concentration of IBA would help in the rapid propagation of 'Marble Queen' cuttings. Applying 500 mg·L^-1 IBA in the oasis medium was more efficient in early rooting and shoot growth of 'Marble Queen' cuttings. Coir dust medium, along with 500 mg·L^-1 IBA, was most suitable for the efficient growth and development of 'Marble Queen' cuttings.

References

Babu, B. H., Larkin, A., & Kumar, H. (2018). Effect of plant growth regulators on rooting behavior of stem cuttings of Terminalia arjuna (ROXB.). Plant Archives, 18(2), 2159–2164. https://doi.org/10.20546/ijcmas.2018.708.250

Cavallari, N., Artner, C., & Benkova, E. (2021). Auxin-regulated lateral root organogenesis. Cold Spring Harbor Perspectives in Biology, 13(7), a039941. https://doi.org/10.1101/cshperspect.a039941

Devi, J., Bakshi, P., Wali, V., Kour, K., & Sharma, N. (2016). Role of auxin and dates of planting on growth of cutting raised plantlets of Phalsa (Grewia asiatica L.). The Bioscan, 11(1), 535–537. https://doi.org/10.5958/0976-4666.2015.00018.2

Elmongy, M. S., Cao, Y., Zhou, H., & Xia, Y. (2018). Root development enhanced by using indole-3-butyric acid and naphthalene acetic acid and associated biochemical changes of in vitro Azalea microshoots. Journal of Plant Growth Regulation, 37, 813–825. https://doi.org/10.1007/s00344-017-9776-5

Gehlot, A., Gupta, R. K., Tripathi, A., Arya, I. D., & Arya, S. (2014). Vegetative propagation of Azadirachta indica: Effect of auxin and rooting media on adventitious root induction in mini-cuttings. Advances in Forestry Science, 1(1), 1–9. https://doi.org/10.14583/2318-7670.v02n04a10

Guan, X., Ye, Q., Li, Z., Zheng, C., & Chen, Q. (2019). The complete chloroplast genome of a variety of Epipremnum aureum ’Neon’(Araceae). Mitochondrial DNA Part B, 4(1), 781–782. https://doi.org/10.1080/23802359.2019.1566790

Kaur, A., & Kaur, A. (2017). Effect of IBA concentrations on success of cuttings of fig cv. Brown turkey. International Journal of Recent Scientific Research, 8(11), 21576–21579.

Kohler, A. E., & Lopez, R. G. (2021). Propagation of herbaceous unrooted cuttings of cold-tolerant species under reduced air temperature and root-zone heating. Scientia Horticulturae, 290, 110485. https://doi.org/10.1016/j.scienta.2021.110485

Kumar, M., Chaudhary, V., & Sirohi, U. (2021). Plant growth regulators and their implication in ornamental horticulture: An overview. International Journal of Agriculture, Environment and Biotechnology, 14(3), 417–445.

Liyanage, A., Nashath, F., Mubarak, M., & Nagoor, A. (2021). Modified Coir Dust Media Elevate the Production of Superior Quality Epipremnum aureum. L Plantlets Derived from Stem Cuttings Modified Coir Dust Media Elevate the Production of Superior Quality Epipremnum aureum. L Plantlets Derived from Stem Cuttings. Sri Lankan Journal of Technology (SLJoT), Special Issue, 34-38.

Markovic, S. (2021). Phytohormone Effect on Pterocephalus depressus Stock Plant Productivity and Phytohormone Accumulation and Movement. (Doctoral dissertation, Colorado State University).

Massoumi, M., Krens, F. A., Visser, R. G., & De Klerk, G.-J. M. (2017). Etiolation and flooding of donor plants enhance the capability of Arabidopsis explants to root. Plant Cell, Tissue and Organ Culture (PCTOC), 130, 531–541. https://doi.org/10.1007/s11240-017-1244-1

Mehta, S., Singh, K., & Harsana, A. S. (2018). Effect of IBA concentration and time of planting on rooting in pomegranate (Punica granatum) cuttings. Journal of Medicinal Plants Studies, 6(1), 250–253.

Meshram, A., & Srivastava, N. (2014). Molecular and physiological role of Epipremnum aureum. International Journal of Green Pharmacy (IJGP), 8(2). https://doi.org/10.4103/0973-8258.129566

Meshram, A., & Srivastava, N. (2015). Epipremnum aureum (Jade pothos): A multipurpose plant with its medicinal and pharmacological properties. Journal of Critical Reviews, 2(2), 21–25.

Moreno, N. H., Álvarez-Herrera, J. G., Balaguera-López, H. E., & Gerhard, F. (2009). Asexual propagation of cape gooseberry (Physalis peruviana L.) using different substrates and auxin levels. Agronomía Colombiana, 27(3), 341–348.

Nasri, F., Fadakar, A., Saba, M. K., & Yousefi, B. (2015). Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.). Journal of Agricultural Sciences, Belgrade, 60(3), 263–275. https://doi.org/10.2298/jas1503263n

Nemati, R., Fortin, J. P., Craig, J., & Donald, S. (2021). Growing mediums for medical cannabis production in north america. Agronomy, 11(7), 1–12. https://doi.org/10.3390/agronomy11071366

Netam, N., Shukla, N., Sharma, G., & Sahu, J. K. (2018). Effect of Different IBA concentration on survivability and rooting of Jasmine (Jasminum sambac (L.) Aiton) stem cuttings. Journal of Pharmacognosy and Phytochemistry, 7(1S), 614–617.

Oni, O., & Ojo, L. (2002). Germination, growth and cloning of the popular West African chewing stick (Massularia acuminata (G. Don) Bullock Ex Hoyle. Nigerian Journal of Ecology, 4(1), 8–12.

Osman, M., Samsudin, N. S., Faruq, G., & Nezhadahmadi, A. (2013). Factors affecting microcuttings of Stevia using a mist-chamber propagation box. The Scientific World Journal, 2013. https://doi.org/10.1155/2013/940201

Osmont, K. S., Sibout, R., & Hardtke, C. S. (2007). Hidden branches: Developments in root system architecture. Annu. Rev. Plant Biol., 58, 93–113. https://doi.org/10.1146/annurev.arplant.58.032806.104006

Overvoorde, P., Fukaki, H., & Beeckman, T. (2010). Auxin control of root development. Cold Spring Harbor Perspectives in Biology, 2(6), a001537. https://doi.org/10.1101/cshperspect.a001537

Pandey, P., Singh, J., Thakur, O., & Bhattacharjee, R. (2019). Effect of different media on the growth, yield and quality of water spinach under container gardening. International Journal of Advanced Research in Biotechnology and Nano Biotechnology, 1(1), 28–38. https://doi.org/10.22161/ijeab.4445

Parthiban, R., Vijayakumar, S., Prabhu, S., & Yabesh, J. G. E. M. (2016). Quantitative traditional knowledge of medicinal plants used to treat livestock diseases from Kudavasal taluk of Thiruvarur district, Tamil Nadu, India. Revista Brasileira de Farmacognosia, 26, 109–121. https://doi.org/10.1016/j.bjp.2015.07.016

Pigatto, G. B., Gomes, E. N., Tomasi, J. de C., Ferriani, A. P., & Deschamps, C. (2018). Effects of indolebutyric acid, stem cutting positions and substrates on the vegetative propagation of Stevia rebaudiana Bertoni. Revista Colombiana de Ciencias Hortícolas, 12(1), 202–211. https://doi.org/10.17584/rcch.2018v12i1.6631

Rajkumar, J. S. G., Kumar, R., Singh, A., & Kumar, A. (2016). Establishment, survival and growth of pomegranate cuttings with different concentrations of indole butyric acid and rooting substrates. Eco. Env. & Cons., 22, 321-327.

Roberto, S. R., & Colombo, R. C. (2020). Innovation in propagation of fruit, vegetable and ornamental plants. Horticulturae, 6(2), 1–8. https://doi.org/10.3390/horticulturae6020023

Samim, A. K., Shivakumar, B., & Ganapathi, M. (2021). Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry. International Journal for Research in Applied Sciences and Biotechnology, 8(5), 147–150.

Sarropoulou, V., Maloupa, E., & Grigoriadou, K. (2023). In vitro direct organogenesis of the medicinal single-mountain local prioritized vulnerable Greek endemic Achillea occulta under different medium variants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(2), 13124–13124. https://doi.org/10.15835/nbha51213124

Wahab, F., Nabi, G., Ali, N., & Shah, M. (2001). Rooting response of semi-hardwood cuttings of guava (Psidium guajava L.) to various concentrations of different auxins. Online Journal of Biological Sciences, 1(4), 184–187. https://doi.org/10.3923/jbs.2001.184.187

Watane, A., Khobragade, Y., Palekar, A., & Singhanjude, A. (2018). Response of marigold cuttings to IBA and media for rooting and growth performance. International Journal of Chemical Studies, 6(5), 1343–1347.