Antibacterial Ingredients That Cause Diarrhea Can Be Made from Black, Green, and White Tea Extract (Camellia sinensis L.)
Abstract
One cause of diarrhea is a bacterial infection. Tea (Camellia sinensis) is a steeping drink that has the efficacy of treating diarrhea. This looks at goals to decide the effect of formulations of three tea extracts that act as antibacterial causes of diarrhea and decide the most appropriate maceration temperature. Extraction was carried out with water solvents at temperatures 70oC, 100oC, and 121oC. The disc method conducted an antibacterial test against E. coli and S. aureus. The most optimal temperature for producing the highest inhibitory zone is the temperature of 100oC. Tea extract temperatures of 100oC are used for antibacterial testing. BSLT test showed the LC50 value of 1800 µg/mL. Tests on E. coli bacteria showed the highest results in a single white tea extract with a diameter of 9.750 mm, and tests on S. aureus showed the highest results in the three tea extract formula with a diameter of 9.65 mm. The inhibitory area evaluation of the system changed into finished using the simplex-centroid design method. It showed a non-huge linear model in the antibacterial test in opposition to E. coli and S. aureus.
References
[AOAC] The Assosiation of Official Analytical Chemistry. (2012). Official Methods of Analysis. Assosiation of Official Analytical Chemistry 19th Edition. Gaitherburg (US): AOAC.
Abdullah, N., & Chin, N. L. (2010). Simplex-centroid mixture formulation for optimised composting of kitchen waste. Bioresource Technology, 101(21), 8205–8210. https://doi.org/10.1016/j.biortech.2010.05.068
Agustina, E., Andiarna, F., Lusiana, N., Purnamasari, R., & Hadi, M. I. (2018). Identifikasi Senyawa Aktif dari Ekstrak Daun Jambu Air (Syzygium aqueum) dengan Perbandingan Beberapa Pelarut pada Metode Maserasi. Biotropic : The Journal of Tropical Biology, 2(2), 108–118. https://doi.org/10.29080/biotropic.2018.2.2.108-118
Agustini, N. wayan S., & Setyaningrum, M. (2017). Identifikasi Senyawa Aktif dan Toksisitas Hayati Ekstrak n-heksana, Etil Asetat dan etanol Mikroalga Tetraselmis chuii Secara Brine Shrimp Lethality Test (BSLT). Warta Industri Hasil Pertanian, 34(1), 8. https://doi.org/10.32765/wartaihp.v34i1.4063
Ahmad Fuzi, S. F., Koller, D., Bruggraber, S., Pereira, D. I. A., Dainty, J. R., & Mushtaq, S. (2017). A 1-h time interval between a meal containing iron and consumption of tea attenuates the inhibitory effects on iron absorption: a controlled trial in a cohort of healthy UK women using a stable iron isotope. The American Journal of Clinical Nutrition, 106(6), 1413–1421. https://doi.org/10.3945/ajcn.117.161364
Almajano, M. P., Carbó, R., Jiménez, J. A. L., & Gordon, M. H. (2008). Antioxidant and antimicrobial activities of tea infusions. Food Chemistry, 108(1), 55–63. https://doi.org/https://doi.org/10.1016/j.foodchem.2007.10.040
Anggraini, T. (2017). Proses dan Manfaat Teh. Padang (ID): Rumahkayu Pustaka Utama.
Arifin, N. B., Marthapratama, I., Sanoesi, E., & Prajitno, A. (2017). Aktivitas Antibakteri Ekstrak Daun Jarak Pagar ( Jatropha curcas Linn ) pada Vibrio Harveyi dan Aeromonas hydrophila Antibacterial Activity of Jatropha curcas ( Linn ) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila. Jurnal Perikanan Universitas Gajah Mada, 19(1), 11–16.
Arshad, M. S., & Batool, S. A. (2017). Natural Antimicrobials, their Sources and Food Safety. In D. N. Karunaratne & G. Pamunuwa (Eds.), Food Additives. IntechOpen. https://doi.org/10.5772/intechopen.70197
Asif, M. (2017). Antimicrobial agents. Journal of Analytical & Pharmaceutical Research, 1(2), 1–5. https://doi.org/10.1016/S1367-5931(97)80006-3
Atma, Y. (2018). Prinsip Analisis Komponen Pangan: Makro dan Mikro Nutrien. In Yogyakarta(ID): Deepublish. https://doi.org/10.21203/rs.3.rs-1019005/v2
BPOM. (2014). Persyaratan Mutu Obat Tradisional. Badan Pengawas Obat Dan Makanan, 1–25.
Buruk Sahin, Y., Aktar Demirtaş, E., & Burnak, N. (2016). Mixture design: A review of recent applications in the food industry. Pamukkale University Journal of Engineering Sciences, 22(4), 297–304. https://doi.org/10.5505/pajes.2015.98598
Buruk Şahin, Y., Demirtas, E., & Burnak, N. (2015). Mixture Design: A Review of Recent Applications in the Food Industry. PAJES. https://doi.org/10.5505/pajes.2015.98598
Chandra, D., Ismono, H., & Kasymir, E. (2013). Prospek Perdagangan Kopi Robusta Indonesia di Pasar Internasional. Jurnal Ilmu Ilmu Agribisnis, 1(1), 110–115.
Chapagai, M. K., Wan Rosli, W. I., Wan Manan, W. M., Jalil, R. A., Karrila, T., & Pinkaew, S. (2017). Effect of domestic cooking methods on physicochemical, nutritional and sensory properties of different varieties of brown rice from Southern Thailand and Malaysia. International Food Research Journal, 24(3), 1140–1147.
Dewi Anjarsari, I. R. (2016). Katekin teh Indonesia : prospek dan manfaatnya. Kultivasi, 15(2), 99–106. https://doi.org/10.24198/kultivasi.v15i2.11871
Dwicahyani, T., Sumardianto, & Rianingsih, L. (2018). Bioactivity Test of Sea Cucumber Holothuria atra Extracts as Antibacterial Agent for Staphylococcus aureus and Escherichia coli. 7(1), 15–24.
Eka Kartika Untari, Sri Wahdaningsih, & Agustia Damayanti. (2014). Efek Fraksi n-Heksana Kulit Hylocereus polyrhizus Terhadap Kadar Malondialdehida Tikus Stres Oksidatif. Pharmaceutical Sciences and Research, 1(3), 154–165. https://doi.org/10.7454/psr.v1i3.3487
Fakheri, B. A., Bagheri, S., & Mahdi Nezhad, N. (2015). Comparison of Antimicrobial and Antioxidant Activities of Four Different Tea Extracts. Journal of Medical Microbiology and Infectious Diseases, 3(3–4), 57–61. http://jommid.pasteur.ac.ir/article-1-101-en.html
Greenwell, M., & Rahman, P. K. S. M. (2015). Medicinal Plants: Their Use in Anticancer Treatment. International Journal of Pharmaceutical Sciences and Research, 6(10), 4103–4112. https://doi.org/10.13040/IJPSR.0975-8232.6(10).4103-12
Harun, N., & Rahmawati, K. A. (2022). The Antioxidant Activity of Extract Combination Juice Ginger Rhizome, Turmeric, Galangal and Kaempferia galanga. 22.
Indonesia, K. R. (2017). Profil Kesehatan Indonesia Tahun 2017. Kemenkes Republik Indonesia. https://doi.org/10.5772/intechopen.69412
Kaban, A. N., Daniel, & LSaleh, C. (2016). Uji Fitokimia, Toksisitas dan Aktivitas Antioksidan Fraksi n-Heksana dan Etil Asetat Terhadap Ekstrak Jahe Merah. Jurnal Kimia Mulawarman, 14(1), 24–28.
Kowalska-Krochmal, B., & Dudek-Wicher, R. (2021). The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance. Pathogens (Basel, Switzerland), 10(2). https://doi.org/10.3390/pathogens10020165
Kurniadi, M., Salam, N., Kusumaningrum, A., Nursiwi, A., Angwar, M., Susanto, A., Nurhikmat, A., Triwiyono, & Frediansyah, A. (2017). Shelf-life prediction of canned “nasi uduk” using accelerated shelf-life test (ASLT) - Arrhenius model. 30096. https://doi.org/10.1063/1.4968349
M, M. V., & Angelina, G. (2014). Extraction And Isolation Of The Alkaloids From The Samanea Saman ( Acacia ) Bark : Its Antiseptic Potential. 3(1), 119–124.
Maslukhah, Y. L., Widyaningsih, T. D., Waziiroh, Elok, Wijayanti, Novita, & Sriherfyna, F. H. (2014). Faktor pengaruh ekstraksi cincau hitam (Mesona palustris BL) skala pilot plan dan Zijp. Jurnal Pangan Dan Agroindustri, 4(1), 245–252. https://doi.org/10.14203/beritabiologi.v17i2.3086
Mclaughlin, J. L., Rogers, L. L., & Anderson, J. E. (1998). Biological Assays to Evaluate Botanicals. Drug Information Journal, 32, 513–524.
Melliawati, R. (2009). Escherichia coli dalam kehidupan manusia. Jurnal Biotrends, 4(1), 10–14. https://doi.org/10.1016/b978-012220751-8/50013-6
Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E. J., & McLaughlin, J. L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica, 45(05), 31-34.
Milanda, T., Lestari, K., & Tarina, N. T. I. (2021). Antibacterial Activity of Parijoto (Medinilla speciosa Blume) Fruit Against Serratia marcescens and Staphylococcus aureus. Indonesian Journal of Pharmaceutical Science and Technology, 8(2), 76. https://doi.org/10.24198/ijpst.v8i2.32166
Mitrowihardjo, S., Mangoendidjojo, W., Hartiko, H., & Yudono, P. (2012). Kandungan Katekin Dan Kualitas (Warna Air Seduhan, Flavor, Kenampakan) Enam Klon Teh (Camellia sinensis (L.) O. Kuntze) di Ketinggian Yang Berbeda. Agritech, 32(2), 199–206.
Moon, K. M., Kwon, E. Bin, Lee, B., & Kim, C. Y. (2020). Recent Trends in Controlling the Enzymatic Browning of Fruit and Vegetable Products. Molecules, 25(12). https://doi.org/10.3390/molecules25122754
Muttaqin, F. Z., Nurul Aida, & Asnawi, A. (2018). ADULTERANT DETECTION ON RAW MATERIAL OF INSTANT TEMULAWAK (Curcuma xanthorrhiza ROXB) PREPARATION USING TLC FINGERPRINT ANALYSIS. Pharmaceutical Journal of Indonesia, 15(1), 38–49. https://ojs.amhinternational.com/index.php/imbr/article/view/2461/1696
Navarre, W. W., & Schneewind, O. (1999). Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiology and Molecular Biology Reviews : MMBR, 63(1), 174–229. https://doi.org/10.1128/MMBR.63.1.174-229.1999
Ngoc Tram, N. (2015). Optimizing the Extraction Conditions of Phenolic Compounds from Fresh Tea Shoot. Journal of Food and Nutrition Sciences, 3(1), 106. https://doi.org/10.11648/j.jfns.s.2015030102.30
Nurjanah, N., Julianti, E. D., & Sahara, E. (2016). Aplikasi Pati Aren Termodifikasi Ekstrak Daun Jambu Biji Merah Dalam Pengembangan Produk Berindeks Glikemik Rendah (Applications of Arenga Starch Modified with Red Guava Leaf Extract in Development of a Low Glycemic Index Product). Nutrition and Food Research, 39(2), 75–86. https://doi.org/10.22435/pgm.v39i2.6969.
Omwenga, O. E., Mariita, R. M., Alaro, L., & Okemo, P. O. (2011). Evaluation of methanolic extracts of six medicinal plants used by herbal practitioners in Central Province-Kenya. 2(4), 867–874.
Othman, L., Sleiman, A., & Abdel-Massih, R. M. (2019). Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Frontiers in Microbiology, 10, 911. https://doi.org/10.3389/fmicb.2019.00911
Ouedrhiri, W., Balouiri, M., Bouhdid, S., Moja, S., Chahdi, F. O., Taleb, M., & Greche, H. (2016). Mixture design of Origanum compactum, Origanum majorana and Thymus serpyllum essential oils: Optimization of their antibacterial effect. Industrial Crops and Products, 89, 1–9. https://doi.org/https://doi.org/10.1016/j.indcrop.2016.04.049
Panawala, L. (2017). Difference Between Gram Positive and Gram negative Bacteria.
Pillet, F., Formosa-Dague, C., Baaziz, H., Dague, E., & Rols, M. P. (2016). Cell wall as a target for bacteria inactivation by pulsed electric fields. Scientific Reports, 6(December 2015), 1–8. https://doi.org/10.1038/srep19778
Praharwati, V. (2015). Perkembangan Perkebunan Teh Jolotigo Kecamatan Talun Kabupaten Pekalongan Tahun 1957-1996 (Pengaruhnya Terhadap Kehidupan Sosial Ekonomi). Journal of Indonesian History, 3(2), 60–64.
Prisinda, D., Setiawan, A. S., Fatriadi, F., & Lestar, D. (2021). Antibacterial Potential Test of Basil Leaf (Ocimum Basilicum) Against Enterococcus Faecalis ATCC 29212. International Journal Of Medical Science And Clinical Research Studies, 01(04), 65–70. https://doi.org/10.47191/ijmscrs/v1-i4-04
Rahman, I. (2016). Comparative analysis of phytochemical constituents, antibacterial and antioxidant activity of green tea (Camellia sinensis). Dhaka (BD): BRAC University.
Ria Kusumaningrum, Agus Supriadi, S. H. R. . (2013). The Characteristics and Quality of Lotus flower (Nelumbo nucifera) tea. Fishtech, 2(1), 9–21.
Rohadi, Lelita, D. I., & Putri, A. S. (2019). Antioxidant capacity of white tea (Camelia Sinensis) extract: Compared to green, oolong and black tea. IOP Conference Series: Earth and Environmental Science, 292(1). https://doi.org/10.1088/1755-1315/292/1/012018
Rohdiana, D., & Al-ghifari, U. (2015). Teh: proses, karakteristik dan komponen fungsionalnya. Foodpreview Indonesia, 10(8), 34–37.
Rohdiana, D., Arief, D. Z., & Budiman, A. (2013). Aktivitas penghambatan pertumbuhan bakteri Escherichia coli oleh berbagai jenis teh dan seduhannya Inhibitory activity of Escherichia coli by type of teas and its liquors. 37–43.
Rohdiana, D., Arief, D. Z., & Somantri, M. (2013). Aktivitas Penangkapan Radikal Bebas DPPH ( 1 , 1-Diphenyl-2-Picrylhydrazyl ) oleh Teh Putih Berdasarkan Suhu dan Lama Penyeduhan. Jurnal Penelitian Teh Dan Kina, 16(1), 47.
Sabathani, A., Widjanarko, S. B., & Yuwono, S. S. (2018). Optimasi Durasi Dan Rasio Bahan Per Pelarut Ekstrak Daun Pepaya Untuk Uji Aktivitas Antibakteri. Jurnal Teknologi Pertanian, 19(3), 193–206. https://doi.org/10.21776/ub.jtp.2018.019.03.6
Sartika, R., Dan, M., & Purwiyanto, A. I. S. (2013). Aktivitas Antibakteri Ekstrak Rumput Laut Eucheuma cottoni terhadap Bakteri Escherichia coli, Staphylococcus aureus, Vibrio cholera dan Salmonella typhosa. Maspari Journal, 5(2), 98–103. http://masparijournal.blogspot.com
Shabri, & Rohdiana, D. (2016). Optimization and characterization of green tea polyphenol extract from various solvents. Jurnal Penelitian Teh Dan Kina, 19(1), 57–66.
Sheela Devi, A., Rajkumar, J., Modilal, M., & Ilayaraja, R. (2012). Antimicrobial activities of Avicennia marina, Caesalpinia pulcherrima and Melastoma malabathricum against clinical pathogens isolated from UTI. International Journal of Pharma and Bio Sciences, 3(3), B698–B705.
Shuyuan, L., Ai, Z., Fengfeng, Q., Yuqiong, C., & Dejiang, N. (2017). Effect of steeping temperature on antioxidant and inhibitory activities of green tea extracts against α-amylase, α-glucosidase and intestinal glucose uptake. Food Chemistry, 234. https://doi.org/10.1016/j.foodchem.2017.04.151
Silhavy, T. J., Kahne, D., & Walker, S. (2010). The bacterial cell envelope. Cold Spring Harbor Perspectives in Biology, 2(5), a000414. https://doi.org/10.1101/cshperspect.a000414
Siregar, A. H. (2017). Pembuatan Zat Warna Alam Dari Tumbuhan Berasal Dari Daun. Bina Teknika, 12(1), 103. https://doi.org/10.54378/bt.v12i1.96
Sugiarto. (2013). Perencanaan unit pengawasan mutu pada pabrik pengolahan teh hitam ctc dengan kapasitas bahan baku 14 ton/hari. niversitas Katolik Widya Mandala Surabaya.
Sukardiman., R. A. dan P. N. F. (2004). Uji praskrining aktivitas anti kanker ekstrak eter dan ektrak metanol Marchantia planiloba Steph. dengan metode uji kematian larva udang dan profil densitometri ekstrak aktif. Majalah Farmasi Airlangga 4, 3(3), 97–100.
Suzuki, M., Sano, M., Yoshida, R., Degawa, M., Miyase, T., & Maeda-Yamamoto, M. (2003). Epimerization of tea catechins and O-methylated derivatives of (-)-epigallocatechin-3-O-gallate: relationship between epimerization and chemical structure. Journal of Agricultural and Food Chemistry, 51(2), 510–514. https://doi.org/10.1021/jf0210627
Theresia, R., Falah, S., Safithri, M., & Assyar, M. (2019). Toxicity Extract and Faction of Surian Toona sinensis Leaf and Bark against Shrimp Larvae Artemia salina L. Current Biochemistry, 3(3), 128–137. https://doi.org/10.29244/cb.3.3.128-137
Towaha, J. (2013). Kandungan senyawa kimia pada daun teh (Camellia sinensis). Warta Penelitian Dan Pengembangan Tanaman Industri, 19(3), 12–16. https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645547325%7B&%7DpartnerID=40%7B&%7Dmd5=5c937a0c35f8be4ce16cb392381256da
Trianes, J., Bastian, B., & Hartati, D. (2022). Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37°C and 25°C. Indonesian Journal of Medical Laboratory Science and Technology, 4(2), 120–127. https://doi.org/10.33086/ijmlst.v4i2.2919
Tsou, L. K., Yount, J. S., & Hang, H. C. (2017). Epigallocatechin-3-gallate inhibits bacterial virulence and invasion of host cells. Bioorganic & Medicinal Chemistry, 25(11), 2883–2887. https://doi.org/10.1016/j.bmc.2017.03.023
Ude, J., Tripathi, V., Buyck, J. M., Söderholm, S., Cunrath, O., Fanous, J., Claudi, B., Egli, A., Schleberger, C., Hiller, S., & Bumann, D. (2021). Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences of the United States of America, 118(31), 1–8. https://doi.org/10.1073/pnas.2107644118
Utami, E. R. (2012). Antibiotika, Resistensi, Dan Rasionalitas Terapi. Sainstis, 1(4), 191–198. https://doi.org/10.18860/sains.v0i0.1861
Utami, N., & Luthfiana, N. (2016). Faktor-Faktor yang Memengaruhi Kejadian Diare pada Anak. Majority, 5, 101–106. https://www.mendeley.com/catalogue/fdd61f29-e548-30b4-9a02-3d11c3c9b4aa/
Vuong, Q. V, Stathopoulos, C. E., Golding, J. B., Nguyen, M. H., & Roach, P. D. (2011). Optimum conditions for the water extraction of L-theanine from green tea. Journal of Separation Science, 34(18), 2468–2474. https://doi.org/https://doi.org/10.1002/jssc.201100401
Wang, Y., Li, J., Zhao, F., Zhang, Y., Yang, X., Lin, Y., & Han, S. (2022). Methanol oxidase from Hansenula polymorpha shows activity in peroxisome-deficient Pichia pastoris. Biochemical Engineering Journal, 180(February), 108369. https://doi.org/10.1016/j.bej.2022.108369
Warnasih, S., & Hasanah, U. (2019). PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw). Journal of Science Innovare, 1(02), 44–49. https://doi.org/10.33751/jsi.v1i02.1000
Wazir, D., Ahmad, S., Muse, R., Mahmood, M., & Shukor, M. Y. (2011). Antioxidant activities of different parts of Gnetum gnemon L. Journal of Plant Biochemistry and Biotechnology, 20(2), 234–240. https://doi.org/10.1007/s13562-011-0051-8
Widyasanti, A., Halimah, T., & Rohdiana, D. (2018). Extraction of White Tea by Ultrasound Assisted Extraction at Various Amplitude. Jurnal Aplikasi Teknologi Pangan, 7(3), 111–116. https://doi.org/10.17728/jatp.2295
Yeni, G., Sa’id, E. G., Syamsu, K., & Mardliyati, E. (2014). Penentuan Kondisi Terbaik Ekstraksi Antioksidan dari Gambir Menggunakan Metode Permukaan Respon. Jurnal Litbang Industri, 4(1), 39. https://doi.org/10.24960/jli.v4i1.637.39-48
Yenita A. (2018). Pengaruh berbagai jenis teh terhadap bioavailabilitas zat besi. IPB University.
Yogi Rabani RS, I. G. A., & Elza Fitriani, P. P. (2022). Analisis Kadar Kafein dan Antioksidan Kopi Robusta (Coffea canephora) Terfermentasi Saccharomyces cerevisiae. Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 11(2), 373. https://doi.org/10.24843/itepa.2022.v11.i02.p18
Yushananta, P., & Usman, S. (2018). The Incidence of Diarrhea in Babies Affected through the Cleanliness of Eating Utensils and Hands. Hand, 70(69), 50-4.
Zayadi, R., Abdul Rahim, N., & Abu Bakar, F. (2016). Determination of Flavonoid and Caffeine Content in Black and Oolong Teas. 18–24.
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