Isolation and Anti-Yeast Activity of Secondary Metabolites of Soursop Leaf Endophytic Fungi

  • Akhmad Endang Zainal Hasan IPB University, Indonesia
  • Dimas Andrianto IPB University, Indonesia
  • Husnawati IPB University, Indonesia
  • Faisal Rahman IPB University, Indonesia
  • Nurliani Bermawie Indonesian Agency for Agricultural Research and Development, Bogor, Indonesia
  • Heddy Julistiono Indonesian Institute of Science, Bogor, Indonesia.
  • Eny Ida Riyanti Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Bogor, Indonesia
DOI: https://doi.org/10.30997/ijar.v3i1.184
Keywords: anti yeast, S.cerevisiae, C.tropicalis, endophytic fungi, ethyl acetate

Abstract

This study aimed to isolate the endophytic fungi of soursop leaves that have the potential as anti-yeast. Soursop leaves were collected from 3 locations in West Java, Indonesia. The secondary metabolites produced by the endophytic fungi were extracted with ethyl acetate and tested for anti-yeast activity against Saccharomyces cerevisiae and Candida tropicalis. Profile of extracts contents characterization was carried out by using thin-layer chromatography (TLC). Twelve monoculture soursop leaves endophytic fungi isolates from Sukabumi (3 isolates), Cianjur (3 isolates), and Garut (6 isolates). It showed different abilities to inhibit C. tropicalis and S. cereviseae. The best extract is isolat from Cianjur (Sir-C1) and Sukabumi (Sir-S1). The stain patterns seen between the nine endophytic fungi extracts were similar. This indicates that the compounds in them may also be the same. But the extracts have a different absorbance for anti-yeast activities. This indicates that the total metabolites of each isolate are different. TLC results showed that ethyl acetate extraction produced almost the same metabolite components from nine endophytic fungi extracts, and all extracts had an anti-yeast activity with various absorbance. There are differences in the ability of 12 soursop leaf endophytic fungi to inhibit yeast model C. tropicalis and S. cerevisiae. Some extracts have similar TLC profiles, but their anti-yeast activity is not similar

References

Adri, D., & Hersoelistyorini, W. (2013). Aktivitas Antioksidan dan Sifat Organoleptik Teh Daun Sirsak ( Annona muricata Linn . ) Berdasarkan Variasi Lama Pengeringan Antioxidant Activity and Organoleptic Charecteristic of Soursop ( Annona muricata Linn .) Leaf Tea Based on Variants Time Drying. Jurnal Pangan Dan Gizi, 04(07).

Agusta, A. (2013). Nerolidol, Komponen Kimia Aromatik Tanaman Teh Yang Juga Diproduksi Oleh Jamur Endofit Schizophyllum SP. D [Nerolidol, an Aromatic Chemical Constituent of Tea Plant That Produces by an Endophytic Fungi Schizophyllum SP. D]. Berita Biologi, 12(2).

Arifni, F. R., Hasan, A. E. Z., Hasim, Julistiono, H., Husnawaty, Bermawie, N., & Riyanti, E. I. (2017). Anti-cancer activities of endophytic fungi isolated from soursop leaves (Annona muricata L.) against WiDr cancer cells. Annual Research and Review in Biology, 18(5), 1–11. https://doi.org/10.9734/ARRB/2017/34657

Audu, S. S., Aremu, M. O., Beetseh, C., Haruna, G. S., & Adoga, J. (2019). Phytochemical screening, antioxidant activity, and mineral composition of soursop (Annona muricata) pulp, peel, and seed. FUW Trends in Science & Technology Journal, 4(2), 501–505. https://doi.org/10.13140/RG.2.2.28043.03367

Backer, R., Rokem, J. S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S., & Smith, D. L. (2018). Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture. Frontiers in Plant Science, 9. https://doi.org/10.3389/fpls.2018.01473

Engel, S. R., Dietrich, F. S., Fisk, D. G., Binkley, G., Balakrishnan, R., Costanzo, M. C., Dwight, S. S., Hitz, B. C., Karra, K., Nash, R. S., Weng, S., Wong, E. D., Lloyd, P., Skrzypek, M. S., Miyasato, S. R., Simison, M., & Cherry, J. M. (2014). The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3: Genes, Genomes, Genetics, 4(3), 389–398. https://doi.org/10.1534/g3.113.008995

Enweani, I. B., Obroku, J., Enahoro, T., & Omoifo, C. (2004). The biochemical analysis of soursop ( Annona muricata L .) and sweetsop ( A . squamosa L .) and their potential use as oral rehydration therapy. Food, Agriculture & Environment, 2(1), 39–43.

Gajalakshmi, S., Vijayalakshmi, S., & Devi Rajeswari, V. (2012). Phytochemical and pharmacological properties of Annona muricata: A review. International Journal of Pharmacy and Pharmaceutical Sciences, 4(2), 3–6.

Hasan, A. E. Z., Bermawie, N., Julistiono, H., Riyanti, E. I., Hasim, H., & Suhendar, U. (2020). Phytochemical screening and anti-breast cancer activities of Annona muricata (L.) leaf extracts. Pharmacologyonline, 2, 23–30.

Lukisari, C., & Harijanti, K. (2010). Penatalaksanaan infeksi Candida tropicalis pada penderita median rhomboid glossitis. Dentofasial, 10(1), 13–18.

Marag, P. S., & Suman, A. (2018). Growth stage and tissue-specific colonization of endophytic bacteria having plant growth-promoting traits in hybrid and composite maize (Zea mays L.). Microbiological Research, 214, 101–113. https://doi.org/10.1016/j.micres.2018.05.016

Minarni, Artika, I. M., Julistiono, H., Bermawie, N., Riyanti, E. I., Hasim, & Hasan, A. E. Z. (2017). Anti-cancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). Asian Pacific Journal of Tropical Medicine, 10(6), 566–571. https://doi.org/10.1016/j.apjtm.2017.06.004

Moghadamtousi, Soheil Z., Kadir, H. A., Paydar, M., Rouhollahi, E., & Karimian, H. (2014). Annona muricata leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-κB. BMC Complementary and Alternative Medicine, 14(1), 1–13. https://doi.org/10.1186/1472-6882-14-299

Moghadamtousi, Soheil Zorofchian, Rouhollahi, E., Karimian, H., Fadaeinasab, M., Firoozinia, M., Abdulla, M. A., & Kadir, H. A. (2015). The chemopotential effect of Annona muricata leaves against azoxymethane-induced colonic aberrant crypt foci in rats and the apoptotic effect of acetogenin annomuricin e in HT-29 cells: A bioassay-guided approach. PLoS ONE, 10(4), 1–28. https://doi.org/10.1371/journal.pone.0122288

Ni Putu Rahayu Artini, Sri Wahjuni, dan W. D. S. (2012). Ekstrak Daun Sirsak (Annona Muricata L.) Sebagai Antioksidan Pada Penurunan Kadar Asam Urat Tikus Wistar. Jurnal Kimia, 6(2), 127–137. https://doi.org/10.1053/j.seminoncol.2004.07.017

Nurrochmat, D. R., Nugroho, I. A., Hardjanto, Purwadianto, A., Maryudi, A., & Erbaugh, J. T. (2017). Shifting contestation into cooperation: Strategy to incorporate different interests of actors in medicinal plants in Meru Betiri National Park, Indonesia. Forest Policy and Economics, 83(January 2015), 162–168. https://doi.org/10.1016/j.forpol.2017.08.005

Paarakh, P., Chansouria, J., & Khosa, R. (2009). Wound healing activity of Annona muricata extract. Journal of Pharmacy Research, 2(3), 404–406. http://herbs.ph/attachments/article/675/Wound healing activity of Annona muricata extract.pdf

Parris, N. A. (1976). Chapter 6 Nature of the mobile phase. In N. A. Parris (Ed.), Instrumental Liquid Chromatography (Vol. 5, pp. 95–126). Elsevier. https://doi.org/https://doi.org/10.1016/S0301-4770(08)61247-6

Polak, B., & Pajurek, E. (2021). Separation of some vitamins in reversed-phase thin-layer chromatography and pressurized planar electrochromatography with eluent containing surfactant. Scientific Reports, 11(1), 1–14. https://doi.org/10.1038/s41598-021-01323-1

Ruta, L. L., Popa, C. V., & Farcasanu, I. C. (2020). Cytotoxicity of Oleandrin Is Mediated by Calcium Influx and by Increased Manganese Uptake in Saccharomyces cerevisiae Cells. Molecules, 25(18), 17–19. https://doi.org/10.3390/molecules25184259

Setyowati, Francisca Murti, W. (2007). Diversity of medicinal plant by Talang Mamak tribe in surrounding of Bukit Tiga Puluh National Park, Riau. Biodiversitas Journal of Biological Diversity, 8(3), 228–232. https://doi.org/10.13057/biodiv/d080313

Solomon-Wisdom. (2014). Phytochemical Screening and Antimicrobial activities of Annona muricata ( L ) leaf extract.

Strobel, G., & Daisy, B. (2003). Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews : MMBR, 67(4), 491–502. https://doi.org/10.1128/MMBR.67.4.491-502.2003

Tan, R.-X., & Zou, W. X. (2001). Endophytes: a rich source of functional metabolites. Natural Product Reports, 18 4, 448–459.

Published
2022-05-12
How to Cite
Hasan, A. E. Z., Andrianto, D., Husnawati, Rahman, F., Bermawie, N., Julistiono, H., & Riyanti, E. I. (2022). Isolation and Anti-Yeast Activity of Secondary Metabolites of Soursop Leaf Endophytic Fungi. Indonesian Journal of Applied Research (IJAR), 3(1), 56-66. https://doi.org/10.30997/ijar.v3i1.184
Issue
Vol. 3 No. 1 (2022): Indonesian Journal of Applied Research (IJAR)
Section
Articles

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