Application of Ojip Chlorophyll Fluorescence Transient Analysis to Study The Postharvest Changes in Photosinthetic Apparatus in Cut Foliage Species
Abstract
Chlorophyll fluorescence (ChlF) is a non-invasive technique that can be potentially used in postharvest research to gain useful information on early responses to postharvest stresses. This study was conducted to validate the application of ChlF transient analysis in determining the postharvest changes in photosynthetic apparatus in three ornamental foliage species, i.e., Cordyline fruticosa ‘Willy’s Gold’ and ‘Rubra’, Dracaena sanderiana ‘White’, and Nephrolepis exaltata. Salicylic acid (100 and 300 mg·L−1), glucose (10 g·L−1), and their combinations were used as holding solutions with control treatment (distilled water) at room temperature (25±2°C). Vase life was evaluated using OJIP analysis. OJIP parameters, i.e., specific energy fluxes per reaction center (ABS/RC, TR/RC, ET/RC, and DI/RC), flux ratios (maximum quantum yield of primary photochemistry-φPo), electron transport efficiency (ψo), and quantum yield of electron transport (φEo), and performance index (PI) were recorded every other day, using a fluorometer (FluorPen 100). Leaf chlorophyll contents of all species and anthocyanin contents of two cordyline cultivars were determined. Data were subjected to ANOVA in a completely randomized design. Mean separation was done by DMRT (p ≤ 0.05). Clear variations in ChlF were observed in every foliage species with the time. OJIP analysis showed species-depended variations. The higher ABS/RC and DI/RC were recorded for D. sanderiana and N. exaltata compared to the PI of those species. At the end of the experiment, the chlorophyll contents were decreased, while anthocyanin contents were increased. Consequently, chlorophyll fluorescence changes in photosynthetic apparatus can be used for the prediction of the postharvest stresses and longevity of cut foliage.
References
Abbott, J. A. (1999). Quality measurement of fruits and vegetables. Postharvest Biology and Technology, 15(3), 207–225.
Alaey, M., Babalar, M., Naderi, R., & Kafi, M. (2011). Effect of pre- and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers. Postharvest Biology and Technology, 61(1), 91–94. https://doi.org/10.1016/j.postharvbio.2011.02.002
Beneragama, C. K., Balasooriya, B., & Perera, T. (2014). Use of OJIP chlorophyll fluorescence transients to probe multiple effects of UV-C radiation on the photosynthetic apparatus of Euglena. International Journal of Applied Sciences and Biotechnology, 2(4), 553–558.
Cocetta, G., & Ferrante, A. (2018). Postharvest application of hydrogen peroxide and salicylic acid differently affects the quality and vase life of cut rose (Rosa hybrida L.) petals and leaves. Advances in Horticultural Science, 32(3), 371–378.
Cocetta, G., Ferrante, A., & Agrarie, S. (2018). Affects the quality and vase life of cut. 32(May), 371–378. https://doi.org/10.13128/ahs-23266
Feild, T. S., Lee, D. W., & Holbrook, N. M. (2001). Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood. Plant Physiology, 127(2), 566–574. https://doi.org/10.1104/pp.010063
Force, L., Critchley, C., & Van Rensen, J. J. (2003). New fluorescence parameters for monitoring photosynthesis in plants. Photosynthesis Research, 78(1), 17–33.
Franco, A. C., Herzog, B., Hübner, C., De Mattos, E. A., Scarano, F. R., Ball, E., & Lüttge, U. (1999). Diurnal changes in chlorophyll a fluorescence, CO2-exchange and organic acid decarboxylation in the tropical CAM tree Clusia hilariana. Tree Physiology, 19(10), 635–644. https://doi.org/10.1093/treephys/19.10.635
Giusti, M. M., & Wrolstad, R. E. (2001). Characterization and measurement of anthocyanins by UV‐visible spectroscopy. Current Protocols in Food Analytical Chemistry, 1, F1-2.
Hassan, F., & Ali, E. (2014). Protective effects of 1-methylcyclopropene and salicylic acid on senescence regulation of gladiolus cut spikes. Scientia Horticulturae, 179, 146–152.
Idirisinghe, I., Rathnayake, R., & Edirisinghe, J. (2013). Current status of postharvest handling of cut flowers and foliage in the retail sites of western and north western provinces of Sri Lanka.
Islam, N., Patil, G. G., & Gislerød, H. R. (2003). Effects of Pre- and Postharvest Conditions on Vase Life of Eustoma grandiflorum (Raf.) Shinn. European Journal of Horticultural Science, 68(6), 272–278.
Ivlev, A. A. (2014). Carbon isotope heterogeneity in photosynthesizing biomass and perspectives of its application in biological studies. Photosynthesis: Functional Genomics, Physiological Processes and Environmental Issues, January 2014, 71–102.
Janda, K., Hideg, É., Szalai, G., Kovács, L., & Janda, T. (2012). Salicylic acid may indirectly influence the photosynthetic electron transport. Journal of Plant Physiology, 169(10), 971–978. https://doi.org/10.1016/j.jplph.2012.02.020
Janda, T., Gondor, O. K., Yordanova, R., Szalai, G., & Pál, M. (2014). Salicylic acid and photosynthesis: Signalling and effects. Acta Physiologiae Plantarum, 36(10), 2537–2546.
Krapp, A., Quick, W. P., & Stitt, M. (1991). Ribulose-1, 5-bisphosphate carboxylase-oxygenase, other Calvin-cycle enzymes, and chlorophyll decrease when glucose is supplied to mature spinach leaves via the transpiration stream. Planta, 186(1), 58–69.
Mota, L. J., Sorg, I., Cornelis, G. R., Parsot, C., Bach, S. J., Stanford, K., McAllister, T. A., Cheng, H., Jiang, N., & Shen, A. (2005). Editorial board• EDITORIAL BOARD. FEMS Microbiology Letters, 252(1).
Pun, U. K., & Ichimura, K. (2003). Role of Sugars in Senescence and Biosynthesis of Ethylene in Cut Flowers. Japan Agricultural Research Quarterly, 37(4), 219–224. https://doi.org/10.6090/jarq.37.219
Ram, M., Prasad, K. V., Singh, S. K., Hada, B. S., & Kumar, S. (2013). Influence of salicylic acid and methyl jasmonate elicitation on anthocyanin production in callus cultures of Rosa hybrida L. Plant Cell, Tissue and Organ Culture, 113(3), 459–467. https://doi.org/10.1007/s11240-013-0287-1
Strasser, R. J., Tsimilli-Michael, M., & Srivastava, A. (2005). Analysis of the fluorescence transient. Analysis of the Fluorescence Transient, 1–47.
Witham, F. H. B. (1971.). Experiments In Plant Physiology/Francis H. Witham, David F. Baydes Androbert M. Devlin.
Yusuf, M., Hayat, S., Alyemeni, M. N., Fariduddin, Q., & Ahmad, A. (2013). Salicylic acid: Physiological roles in plants. In Salicylic acid (pp. 15–30). Springer.
Zamani, S., Kazemi, M., & Aran, M. (2011). Postharvest life of cut rose flowers as affected by salicylic acid and glutamin. World Applied Sciences Journal, 12(9), 1621–1624.
Copyright (c) 2021 Indonesian Journal of Applied Research (IJAR)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright publishing of the article shall be assigned/transferred to Indonesian Journal of Applied Research (IJAR) Universitas Djuanda as Publisher of the journal. Upon acceptance of an article, authors will be asked to complete a 'Copyright Transfer Agreement'. An e-mail will be sent to the corresponding author confirming receipt of the manuscript together with a 'Copyright Transfer Agreement' form by online version of this agreement.
Indonesian Journal of Applied Research (IJAR) Universitas Djuanda, the Editors and the Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Indonesian Journal of Applied Research (IJAR) Universitas Djuanda are sole and exclusive responsibility of their respective authors and advertisers.
Remember, even though we ask for a transfer of copyright, our journal authors retain (or are granted back) significant scholarly rights as mention before.
The Copyright Transfer Agreement (CTA) Form can be downloaded here: Copyright Transfer Agreement-IJAR 2020
The copyright form should be signed electronically and send to the Editorial Office e-mail below:
Prof. Dr. Ir. Dede Kardaya, M.Si. (Editor-in-Chief)
Universitas Djuanda
Jl. Tol Jagorawi No.1, Ciawi, Kec. Ciawi, Bogor, Jawa Barat 16720
Website: http://journal.unida.ac.id/index.php/IJAR/index
Email: ijar@unida.ac.id